Quercetin is a flavonoid and is a major constituent of the species Ipomoea sepiaria koenig ex. Roxb. Analytical thin layer chromatography was carried out on silica gel GF254 TLC plates and the chromatoplate was developed with chloroform:acetone:formic acid (76:16.5:85 v/v) as mobile phase. An RP-HPLC method was developed for the estimation of quercetin from of Ipomoea sepiarea. Mobile phase composed of acetonitrile: methanol: triethyle amine (45:55:0.5% v/v). The flow rate was 1.2 ml/min and the analyses of column effluents were performed using uv-visible detector at 264 nm. Retention time of quercetin was found as 1.5 min. This method has obeyed linearity over the concentration range of 2-10 µg/ml and the regression coefficient obtained from linearity plot for quercetin was found as 0.999. RP-HPLC method was validated in pursuance of ICH guidelines.
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